extraction d or kelantan

Efficacy of DNA Extraction Methods Using Simple …

Nonetheless, DNA (Deoxyrebonucleic acid) extraction is always a limiting factor. In this study, we analysed human microbes to compare the performance of DNA extraction methods: simple boiling method and MasterPureTM Complete DNA and RNA Purification Kit.

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OPTIMIZATION OF REBAUDIOSIDE A EXTRACTION FROM …

2013-6-23 · OPTIMIZATION OF REBAUDIOSIDE A EXTRACTION FROM STEVIA REBAUDIANA (BERTONI) AND QUANTIFICATION BY HIGH PERFOMANCE LIQUID CHROMATOGRAPHY ANALYSIS ASRUL AFANDI*, SHAZANI SARIJAN AND RANAJIT KUMAR SHAHA Faculty of Agro Based Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked Bag No. 100, 17600 Jeli, Kelantan…

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Preliminary Study of Buffer Ratio in Protein Extraction ...

2020-12-1 · Protein extraction is a preliminary step of protein purification which mainly focus on maximization of total protein yield. The heterogeneous properties cause diversification of protein; therefore, there is no absolute protocol in protein extraction. The ratio of buffer gives different protein concentrations in different types of mammalian tissues, and this condition leads to the study of ...

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Determination of Extraction Buffer and Ammonium …

2020-7-6 · sugar was quantified using D-galacturonic acid as stand-ard. The enzyme activity was calculated as the amount of the enzyme required to release one micromole equivalent of D-galacturonic acid per min under assay condition. Finally, the supernatant that was proven to have enzyme activity was passed through a Millipore filter membrane (0.45µm), lyo-

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EXTRACTION OF ESSENTIAL OIL FROM CURCUMA …

Ethanol has been used as solvent to extract the curcumin. Rotary evaporator was used to purify the sample. The samples were put in rotovap at temperatures 40°C, 50°C, 60°C and 70°C. HPLC analysis showed that all of the samples had high percentage of curcumin.

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Potential applications of enzymes on the extraction of ...

Samples of Mas Cotek leaves extracts from each extraction method were first analysed using HPLC once the extraction was done. Vitexin compound in this fine extract were identified and quantified by using a reversed-phase Phenomenex (Torrance, CA, USA) column (Prodigy, C 18, 5 µ, 250 × 4.60 mm i.d.). Calibrating curves of

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Potential of the Biodegradability and Characteristics of ...

D. N. Roma Advance Industrial Biotechnology Cluster (AdBiC), Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, Malaysia

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Antiproliferative effect of methanolic extraction of ...

2011-9-26 · Background. Scar formation is the result of natural healing process which occurs after wounds, trauma, burns, surgical incision or disease. In order to close a wound, skin repair itself from normal to scar tissue and thus prevent infection [].Keloid is an end of the full spectrum of scar which extends beyond the boundaries of the original wound.

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Efficacy of DNA Extraction Methods Using Simple …

The rapid progression of molecular-based technology now enables us to analyse huge number of samples. Nonetheless, DNA (Deoxyrebonucleic acid) extraction is always a limiting factor. In this study, we analysed human microbes to compare the performance of DNA extraction methods: simple boiling method and MasterPureTM Complete DNA and RNA Purification Kit.

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High Quality of Bacterial DNA Extraction from …

Corbicula fluminea served as a popular traditional food in Kelantan, Malaysia. This clam is highly consumed by the local people from the last decade. However, there is lack of the study that obtain high quality of bacterial metagenome deoxyribonucleic acid (DNA) extraction from C. fluminea. Improper DNA extraction protocol always fails to extract a high integrity of bacterial DNA bands.

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Solvent Extraction and Stripping of Silver Ions in …

2019-5-25 · We found that a calix[4]arene-bearing pyridine is soluble in a typical room-temperature ionic liquid (RTIL), 1-alkyl-3-methylimidazolium hexafluorophosphate. Pyridinocalix[4]arene showed a high extraction ability and selectivity for silver ions. The extraction performance of the calix[4]arene was greatly enhanced by dissolution in RTILs compared to in chloroform.

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A review on the extraction methods use in ...

2019-6-24 · s tau d ipl y ;7- mcrow ve er microwave oven; 8-flask; 10-base of flask (Wang et al. 2009) f) Percolator and its schematic picture g) 1 mL stainless steel cells and schematic diagram of packed extraction cell with layers of cellulose filter paper, sand and mixture of sample and sand [29]. Figure 1: (a-g) Drying and extraction equipment. Figure ...

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Preliminary Study of Buffer Ratio in Protein Extraction ...

Preliminary Study of Buffer Ratio in Protein Extraction from Placental Cotyledons of Kedah-Kelantan Cattle Y. Y. Teea, K. Mata,b,*, M. A. Adam a, N. D. Rusli a,b, C. H. Hasnita, & H. N. Mohd Khalida aFaculty of Agro-Based Industry, Universiti Malaysia Kelantan, ... cattle Kedah Kelantan in Agropark, University Malaysia Kelantan Jeli Campus. The ...

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Kelantan

2021-3-11 · Kelantan (Malay pronunciation: [kəˈlantan]; Jawi: کلنتن; Kelantan Malay: Kelaté) is a state of Malaysia.The capital is Kota Bharu and royal seat is Kubang Kerian.The honorific name of the state is Darul Naim (Jawi: دار النعيم, "The Blissful Abode"). Kelantan is located in the north-eastern corner of the peninsula. Kelantan, which is said to translate as the "Land of Lightning ...

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Characteristics and cadmium extraction performance of …

2018-4-18 · 1 SHORT TITLE: PVC/Aliquat electrospun fibres for Cd 2 extraction 3 FULL TITLE: Characteristics and cadmium extraction 4 performance of PVC/Aliquat 336 electrospun fibres in 5 comparison with polymer inclusion membranes 6 7 Submitted to 8 Separation Science and Technology 9 10 March 2015 11 Nurul Syazana Abdul-Halim1,2, Philip G. Whitten3 and Long D. Nghiem2,*

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enrichissement de minerai d''or en Inde

broyeur à barres pour l''extraction de minerai d''or. ... Obtenez le prix en ligne » La guerre populaire en Inde ... fer usine d''enrichissement du minerai en ...

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Bioindustrial Technology

2  · In conventional extraction, there are two parameters studied namely; extraction time (4, 6, and 8 hours) and temperature (30℃, 40℃, 50℃ and 60℃). From the obtained results of conventional extraction, the optimum yield of tannic acid was found at 6 hours of extraction time with temperature of 50℃ was 0.1527 mg/mL.

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Extraction of Essential Oil from Cinnamomum zeylanicum

2017-8-27 · distillation was lower than Soxhlet extraction''s. Higher temperature caused the solvent to evaporate faster. Soxhlet extraction used ethanol as the solvent which had lower boiling point than water and ethanol was a volatile substance. Since the product was oil, some of it stuck to the collection jar during extraction and some was

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Extraction, characterization and molecular structure of ...

2020-4-13 · Bovine skin was incubated with plant enzymes bromelain (B) and zingibain (Z) at the level of 0, 5, 10, 15, 20 and 25 unit/g of skin and gelatin was extracted at 60 °C for 6 h. Control gelatin was extracted without enzymatic pretreatment. The yield and gel strength were 17.90% and 283.35 g for the control samples and 22.26% and 160.88 g for B20 samples. The zingibain extracted gelatin (GEZ ...

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Optimised Production and Extraction of Astaxanthin from ...

2020-3-19 · extraction method (DMSO/acetone). When supercritical CO2 was employed as an extraction solvent in Accellerase-pre-treated Xanthophyllomyces dendrorhous cells, astaxanthin extraction increased 2.5-fold. Overall, the study showed that extraction conditions can be tailored towards targeted pigments present in complex mixtures, such as in microbial cells.

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Extraction of Essential Oil from Cinnamomum zeylanicum

2017-8-27 · Soxhlex extraction. Steam distillation produced high quality essential oil extraction using separatory funnel. Soxhlet extraction produced essential oil in crude form using rotary evaporator to purify the extracted product. Cinnamon essential oil contains high cinnamaldehyde content which is the main component in cinnamon.

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Gut microbiome of pre-adolescent children of two ...

2019-5-24 · As for the weighted PCoA plots (Fig. 1b,d), there was a separation between the Guangzhou and Kelantan Chinese (p = 0.001, R = 0.260) but there was no separation between the Guangzhou and Penang ...

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Research Article Efficiency of Polyphenol Extraction from ...

2019-7-30 · Research Article Efficiency of Polyphenol Extraction from Artificial Honey Using C 18 Cartridges and Amberlite XAD-2 Resin: A Comparative Study ChuaYungAn, 1 Md.MuradHossain, 2 FahmidaAlam, 3 Md.AsifulIslam, 3 Md.IbrahimKhalil, 3,4 NadiaAlam, 3 andSiewHuaGan 3 Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, …

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Nested allele-specific multiplex polymerase chain …

Rasmaizatul Akma Rosdi 1, Nurfadhlina Musa 2, Zalina Zahari 3, Mohd Khairi Zahri @ Johari 4, Mulham Alfatama 3 and Boon Yin Khoo 5. 1 School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia. 2 Human Genome Center, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.

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Determination of Extraction Buffer and Ammonium …

2020-7-6 · Table 1: Type of extraction buffer and percentage of Ammonium sulphate precipitation recommenda-tion for each genera Mangifera, Durio and Syzygium Species Extraction Buffer Ammonium sulphate pre - cipitation (%) Mangifera indica Tris-HCl 0.5M pH 6.4 80 Mangifera odorata Tris-HCl 0.2M pH 7.8 85 Durio brunei Phosphate buffer saline (PBS) 0.05 pH 6.8 80

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Comparison of ten different DNA extraction …

DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely ...

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EXTRACTION OF THE ESSENTIAL OIL OF AQUILARIA …

2015-3-3 · hydro-distillation and solvent extraction. For hydrodistillation, the lab grade sample of agarwood distilled to obtain essential oil will be compared with previously procured samples from MAHA and Kelantan to examine differences in composition. In the solvent extraction procedure, three solvents used hexane, dichloromethyl and –

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[PDF] Solvent Extraction and Stripping of Fe and Mn …

DOI: 10.3303/CET1757190 Corpus ID: 13193097. Solvent Extraction and Stripping of Fe and Mn from Aqueous Solution Using Ionic Liquids as Extractants @article{Ola2017SolventEA, title={Solvent Extraction and Stripping of Fe and Mn from Aqueous Solution Using Ionic Liquids as Extractants}, author={P. D. Ola and Y. Kurobe and M. Matsumoto}, journal={Chemical engineering transactions}, …

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Optimized Extraction Condition and Characterization …

Also, sun-dried and microwave-dried peels were studied at the same extraction time 1 hour at 1.5 pH. Pectin yields varied from 10.4% to 59.30% for sun dried peel while for microwave dried peel was 25.9% to 61.80%. The best extraction condition is using citric acid at 90 ℃ using microwave-dried peels.

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Solvent Extraction and Stripping of Silver Ions in …

2004-7-10 · We found that a calix[4]arene-bearing pyridine is soluble in a typical room-temperature ionic liquid (RTIL), 1-alkyl-3-methylimidazolium hexafluorophosphate. Pyridinocalix[4]arene showed a high extraction ability and selectivity for silver ions. The extraction performance of the calix[4]arene was greatly enhanced by dissolution in RTILs compared to in chloroform. In a competitive extraction ...

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Dental caries experience of elderly people living in ...

2017-9-19 · Archives of Orofacial Sciences (2007) 2, 20-25 20 ORIGINAL ARTICLE Dental caries experience of elderly people living in "Pondok" in Kelantan Seman Ka*, Abdul Manaf Hb, Ismail ARc a Gua Musang Dental Clinic, 18300 Gua Musang, Kelantan, Malaysia. b Department of Community Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, …

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